A new look at how genes unfold to enable their expression

(PhysOrg.com) -- Cornell researchers have uncovered surprising new information about the process by which genes are unwrapped and exposed so that they can be expressed.

There are 3 billion base pairs of DNA in the human genome, stored in compacted structures in order to fit into the nucleus of a cell. The DNA strands wrap twice around central histone proteins, like rope wrapped around a spool, to create packets called nucleosomes, which then assemble into larger and still more compact structures.

When a cell needs a particular protein, the code for it must be transcribed from a strand of DNA by a large, complex enzyme called RNA polymerase II, which copies the code onto a strand of RNA that carries instructions for the cell to produce the protein. To allow this, the nucleosomes disassemble, exposing the part of the DNA strand that is to be copied.

Scientists have long suspected that RNA polymerase II itself drives the unwrapping of DNA from its normal tightly packaged state, but a new study published in the July 11 issue of the journal Cell offers a quite different view. It presents evidence that DNA unwinds from its tight packing even before the transcription enzyme makes contact with that DNA and begins to read it. "Somehow a signal independent of the RNA polymerase II opens up the nucleosome," said John Lis, the paper's senior author and the Barbara McClintock Professor of Molecular Biology and Genetics at Cornell.

While this process has been hinted at in previous studies, this is the first study to offer detailed molecular evidence of bound DNA disassembling independent of RNA polymerase II. The researchers suspect that what they have found holds true for many highly activated genes -- those that are called upon to manufacture large quantities of proteins.

"The paper is a molecular dissection of a process we have observed with a microscope at lower resolution as puffs," Lis said. "This new mechanism is so rapid that it is rather startling. It's not the way we normally think about how transcription occurs."

"This study is exciting because everyone always believed these changes in nucleosomes were a result of active transcription and this research shows that transcription and disassembly of nucleosomes can be uncoupled from each other," added Steven Petesch, the paper's lead author and a graduate student in Lis' lab.

Petesch and Lis used heat shock genes from fruit flies as a model system. Heat shock genes are present in many organisms and become activated when temperatures rise above a threshold, as with fevers in humans and on a hot day for fruit flies. These genes activate processes that protect cells from damage, and even play a role in cancer. Researchers can turn on transcription of these genes in seconds by heating. When it is turned on, all the cells in a sample respond together, making it easy to watch processes unfold.

After applying heat, the researchers chemically froze the transcription process at a series of times separated by seconds, and at each stage probed the sample with an enzyme that cuts DNA that is not assembled into a nucleosome. This allowed them to track when and where the unpacking of nucleosomes occurred, and they found that the unpacking occurred well in advance of the arrival of the transcription enzyme.

Additionally, by studying which sections of DNA were cut during their experiment, they found that "This unpacking of nucleosomes is limited by distinct barriers, which protect neighboring genes that lie beyond these barriers," said Lis. This limits the disruption of nucleosomes to only a short section of the DNA around the gene that needs to be expressed.

"We've only tested this at heat shock genes," said Petesch. "But we wouldn't be surprised if it is used by other genes."

The research was funded by a grant from the National Institutes of Health.

Provided by: Cornell University

Related stories:

Genes that control embryonic stem cell fate identified
Scientists have identified about two dozen genes that control embryonic stem cell fate. The genes may either prod or restrain stem cells from drifting into a kind of limbo, they suspect. The limbo lies between the embryonic stage and fully differentiated, or specialized, cells, such as bone, muscle or fat.
Scientists identify key roadblock to gene expression
A team of scientists has provided, for the first time, a detailed map of how the building blocks of chromosomes, the cellular structures that contain genes, are organized in the fruit fly Drosophila melanogaster. The work identifies a critical stop sign for transcription, the first step in gene expression, and has implications for understanding how the AIDS virus regulates its genes. The findings will be published in the 15 May 2008 issue of the journal Nature.
University of Toronto scientists map entire yeast genome
University of Toronto scientists have devised a tool to help understand and predict the state of a cell by successfully mapping all 70,000 nucleosomes in yeast. Nucleosomes wrap DNA before it is transformed into proteins and are critical indicators and regulators of a cell’s state.
Researchers identify how to switch off cancer cell genes
A new study led by researchers at the University of Southern California (USC) identifies how genes are silenced in cancer cells through distinct changes in the density of nucleosomes within the cells.
Chromatin remodeling complex connected to DNA damage control
When molecular disaster strikes, causing structural damage to DNA, players in two important pathways talk to each other to help contain the wreckage, scientists at The University of Texas M. D. Anderson Cancer Center report in the August edition of Cell.
Gene-transcription machinery seen poised for action, held in check until needed
For some time, scientists have been tracking down the sequence of biochemical steps required to attract and assemble at the head end of a gene the molecular machinery needed to transcribe that gene to put to work the information it encodes. Now, a new study led by researchers at The Wistar Institute suggests that the gene-transcription machinery, once in place, can remain poised for action but held in check until a triggering signal sends it on its way down the linear DNA molecule.
Researchers identify a process that enables access to genes
It turns out there’s more than one way to skin a gene. New research from Rockefeller University suggests that two closely related DNA unpackaging mechanisms may not work the way scientists thought.
Scientists reveal structure of gateways to gene control
Scientists at Penn State University will reveal in the 29 March 2007 issue of the journal Nature the first complete high-resolution map of important structures that control how genes are packaged and regulated throughout an entire genome. "For the first time, we are seeing in very high resolution on a genome-wide scale how nucleosomes control the expression of an organism's genes," said B. Franklin Pugh, professor of biochemistry and molecular biology and the study's lead investigator.

News discussion:

General Science news